Spatial transcriptomics

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Spatial transcriptomics

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Spatial transcriptomics

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Spatial transcriptomics

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Spatial transcriptomics

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Spatial transcriptomics

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Spatial transcriptomics

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Spatial transcriptomics

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Spatial transcriptomics

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Spatial transcriptomics

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Spatial transcriptomics

Click here

What is Spatial transcriptomics ?

Spatial transcriptomics is a range of methods designed for assigning cell types to their locations in the histological sections.

Spatial transcriptomics

Click here

It provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and valuable in research and diagnostics.

Spatial transcriptomics

Click here

What is Spatial Biology?

Spatial Biology is defined as the study of tissues within their own 2D or 3D context. It is the new frontier of molecular biology.

Spatial transcriptomics

Click here

How does spatial Transcriptomics work?

Spatial Transcriptomics utilizes spotted arrays of specialized mRNA-capturing probes on the surface of glass slides. ....

Spatial transcriptomics

Click here

.....
Each spot contains capture probes with a spatial barcode unique to that spot. When tissue is attached to the slide, the capture probes bind RNA.

Spatial transcriptomics

Click here

Why is spatial profiling such a powerful technique?

Provides information of cell type composition and provides the genomic information of single cells.

Spatial transcriptomics

Click here

Commercialized techniques such as Spatial Transcriptomics , released as Visium by 10X Genomics, as well as GeoMx and CosMx by Nanostring

Spatial transcriptomics

Click here

Best-practice analysis pipeline:
1. Normalization and Quality Control
2.cell deconvolutio
3. Spot resolution enhancemen
4. Dimensionality reduction

Spatial transcriptomics

Click here

....
5. Integration with scRNA-seq data
6. Label transfer from scRNA-seq data
7. Clustering of the spots
8. Visualization of clusters

Spatial transcriptomics

Click here

The pipeline combines multiple tools, toolkits and platforms:
Bioconductor
Seurat
scran
scanpy
anndata
scanorama
SpatialExperiment

Spatial transcriptomics

Click here

What is Spatial transcriptomics ?Spatial transcriptomics is a range of methods designed for assigning cell types to their locations in the histological sections.

Spatial transcriptomics

Click here

It provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and valuable in research and diagnostics.

Spatial transcriptomics

Click here

What is Spatial Biology?Spatial Biology is defined as the study of tissues within their own 2D or 3D context. It is the new frontier of molecular biology.

Spatial transcriptomics

Click here

How does spatial Transcriptomics work?Spatial Transcriptomics utilizes spotted arrays of specialized mRNA-capturing probes on the surface of glass slides. ....

Spatial transcriptomics

Click here

.....Each spot contains capture probes with a spatial barcode unique to that spot. When tissue is attached to the slide, the capture probes bind RNA.

Spatial transcriptomics

Click here

Why is spatial profiling such a powerful technique?Provides information of cell type composition and provides the genomic information of single cells.

Spatial transcriptomics

Click here

Commercialized techniques such as Spatial Transcriptomics , released as Visium by 10X Genomics, as well as GeoMx and CosMx by Nanostring

Spatial transcriptomics

Click here

Best-practice analysis pipeline: 1. Normalization and Quality Control 2.cell deconvolutio3. Spot resolution enhancemen4. Dimensionality reduction

Spatial transcriptomics

Click here

....5. Integration with scRNA-seq data6. Label transfer from scRNA-seq data7. Clustering of the spots 8. Visualization of clusters

Spatial transcriptomics

Click here

The pipeline combines multiple tools, toolkits and platforms:Bioconductor Seurat scran scanpy anndata scanorama SpatialExperiment

What is Spatial transcriptomics ?

Spatial transcriptomics is a range of methods designed for assigning cell types to their locations in the histological sections.

What is Spatial Biology?

Spatial Biology is defined as the study of tissues within their own 2D or 3D context. It is the new frontier of molecular biology.

How does spatial Transcriptomics work?

Spatial Transcriptomics utilizes spotted arrays of specialized mRNA-capturing probes on the surface of glass slides. ....

.....
Each spot contains capture probes with a spatial barcode unique to that spot. When tissue is attached to the slide, the capture probes bind RNA.

Why is spatial profiling such a powerful technique?

Provides information of cell type composition and provides the genomic information of single cells.

Best-practice analysis pipeline:
1. Normalization and Quality Control
2.cell deconvolutio
3. Spot resolution enhancemen
4. Dimensionality reduction

....
5. Integration with scRNA-seq data
6. Label transfer from scRNA-seq data
7. Clustering of the spots
8. Visualization of clusters

The pipeline combines multiple tools, toolkits and platforms:
Bioconductor
Seurat
scran
scanpy
anndata
scanorama
SpatialExperiment